Cloning the Cheetah: Why Bother?

In an attempt to discover why the cheetah has such a low fertility rate, a team of scientists, including David Wildt and Stephen O'Brien, decided to take a look at blood samples from a number of cheetahs, labeled "Vulnerable" by the IUCN.  What they found was startling, and very worrying; the cheetahs appeared to be dangerously, perilously inbred; they were all practically clones of each other. 

Wildt and O'Brien decided to confirm this hypothesis by taking skin samples from a number of cheetahs, and attempting to graft them onto a number of other cheetahs.  In a healthy population of animals, the skin grafts would be immediately rejected.  This is because the body of the animal who received the skin graft would recognize the skin to be from another individual, a foreign body, and attack it, just as it would attack any foreign body (i.e., germs or bacteria.)  Even in humans, skin grafts are often rejected, even ones from close relatives.

Normally a very majestic looking animal, this particular cheetah seems to have been caught at a bad time.  Photo Credit: Ted and Gail Neher

So when every single one of the cheetah skin grafts were accepted by the other animals, this did not bode well for the cheetah.  This meant the genetic diversity of the cheetah was incredibly, shockingly low.  But why is genetic diversity so important?  Well, without genetic diversity, the DNA of the individuals comprising a given population are very similar.  These individuals essentially become clones of each other, with many similar attributes, most important among them their immune systems.  In a healthy population of, say, cheetahs, say that fifty percent of them are immune to a certain disease, most of them evolving from a common ancestor.  The other fifty percent of the population has absolutely no protection against said disease, and the illness ravages their population.  An unfortunate (and oversimplified) event, to be sure, but fifty percent of the individuals remain to rebuild the population to what it once was.  With the low genetic diversity seen in the cheetah, if a similar disease in the hypothetical above sweeps through Africa, it is quite likely that a much lower percent than fifty would survive.  It is also possible that this disease would so ravage the global population of cheetahs that they would simply be unable to recover from this catastrophic event.

A cheetah scratching a log at the Denver Zoo.  The cheetahs incredibly slender build can help distinguish it from other cats. 

So why is the cheetah so catastrophically inbred?  Most of the time, low genetic diversity is due to a given species having passed through a "bottleneck."  20,000 years ago, cheetahs spanned the entire globe; ranging all over Europe, Asia and Africa, two species of cheetah, or "proto-cheetahs" if you will, even roamed North America (Miracinonyx inexpectatus and M. trumani.)  When the most recent Ice Age drew to a close around 10,000 years ago, the dramatic climatic changes (and likely human influences, as well) resulted in the extinction of much of the world's mammalian megafauna. Researchers think that this mass-extinction managed to knock the cheetah's numbers down to a very small population, likely comprising a mere handful of individuals.  (In fact, I remember reading once that at one time, some people thought that a single pregnant female mother was able to survive, but I don't think that this idea is all that highly regarded anymore).  While the cheetahs were obviously able to build their population numbers and density by a significant amount than that seen 10,000 years ago, this bottleneck came with a price; genetic diversity was, and is, miniscule.

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Smilodon Vs. Thylacosmilus: The Saber-Toothed Marsupial

Two million years ago, a saber-toothed predator stalked the landscape of South America.  Possessed with long, dagger-like teeth, Thylacosmilus was undoubtedly a terrifying predator of the plains of Patagonia.  This saber-toothed predator would have given even the saber-toothed cats pause, despite the fact that (to paraphrase Obi-Wan Kenobi), "He's more kangaroo now than cat."  Despite superficial similarities, Thylacosmilus was not a saber-toothed cat.  Instead, it was a six foot long, 500 pound saber-toothed marsupial.

A reconstruction of Thylacosmilus by the talented young artist Sam Lippincott.  Interestingly, the super-sized canines of Thylacosmilus grew continually throughout its life, unlike those of Smilodon or Xenosmilus (see below), two of the actual saber-toothed cats.  Photo Credit: Sam Lippincott

The immense canines possessed by Thylacosmilus had previously evolved in both the cat-like Nimravids and the various saber-toothed cats (amongst others), and is a classic example of convergent evolution, a topic, in my opinion, that is one of the most interesting happenstances in nature.  We will hopefully talk about convergent evolution sometime next week.

A mounted skeleton of Xenosmilus, a type of saber-toothed feline, at the Mace Brown Museum of Natural History at the College of Charleston in South Carolina.  I got to visit here in July of 2014, and it was a very nice little museum.  Compare the skull of Xenosmilus with that of Thylacosmilus (below).

Anyways, Thylacosmilus lived during the Miocene and the Pliocene Epochs, from 10-2 MYA.  Up until 2 MYA, South America had been its own, separate land mass, not connected to any other continents since some time during the Cretaceous.  2 MYA, however, something extraordinary happened: the Isthmus of Panama was formed, connecting the two continents.  With this connection, came something scientists have dubbed the "Great American Interchange."  Animals from both continents could move, and spread out into the other continents.  For some creatures, like the saber-toothed cats, this was a good thing; they moved down into South America from North America and dominated the landscape.  For other predators, like the terror-bird Titanis(again, a topic for another time), it was good, for a while; after moving into the southern part of North America, however, Titanis was outcompeted by other predators.  For Thylacosmilus, it was down-right disastrous.  Shortly after the Great American Interchange, fossil evidence of Thylacosmilus entirely disappears, similar to the competition between the dingo and the thylacine that drove the thylacine to extinction on mainland Australia.

The skull of Thylacosmilus on display at the American Museum of Natural History in New York.  Got to see this sucker in person in August 2014, when I visited with my good buddy Zach Evens! 

This is a partial post for the "Convergent Evolution" series.  That means that this post is partially included, but was not made specifically to be a part of that series.  HERE is a link to the Homebase for this series.